生醫所103-1專題演講活動，敬邀 國立台灣大學 生化科技系 陳進庭教授進行專題演講

演講主題：Photodynamic therapy: translational studies and epigenetic regulation

Abstract：

Photodynamic therapy (PDT) has been used for the treatment of superficial tumors due to its limited light penetration. The anticancer drug cisplatin (cDDP) is in widespread use for the cancer; however, the clinical use of cDDP is restricted by severe side effects. In this study, we established a polyethylene glycol (PEG) modified liposome which has Ce6 encapsulated in the lipid bilayer and cDDP in the aqueous interior. The particle size of this Lipo-Ce6-cDDP was about 155 nm. Under the light irradiation, Ce6 was activated to generate photodynamic effect that further promotes the release of cDDP to enhance the cytotocixity of cancer cells. We also examined the anti-tumor efficacy of this dual-effect liposome (Lipo-Ce6-cDDP) in the BALB/cByJ mice bearing C-26 cells. Our results indicate that one dose of dual-effect liposome could be used to completely remove C26 tumor with size around 100~300 mm3. When the cDDP dose was increased to 5.71 mg/kg, it could be used to treat C26 tumor with size of 500 mm3.

Previously, we have established PDT-derived variants from human melanoma A375 cells. The established variants have phenotypic morphology alteration and metastatic potential. Further studies showed that the decreased invasiveness of cancer cells induced by PDT-mediated damage relates to the down-regulation of CLIC4 and its subsequent MMP9 expression. The role of PDT-induced epigenetic modification on CLIC4 will be further discussed.